FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2633502
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG6500.041.211183
CTACTCG6550.040.1958962
CCCAAAG46600.039.9768261
AAAGTGC38300.037.0562744
GCTGGGA67500.036.7606939
GCTACTC16550.036.6682971
CCAAAGT45100.036.2775272
AGTAGCT28300.035.8691335
CGAGTAG7200.035.2464033
AAGTGCT41850.034.3620875
AGTGCTG43150.033.87146
CAAAGTG45850.033.4142573
GTGCTGG45100.033.136317
GGAGGCT51000.032.9887929
GAGTAGC18200.032.7933584
CCGAGTA8100.031.923662
TTGGGAG44400.031.4359846
TAGCTGG34500.030.7852677
GTAGCTG31650.030.7362026
CTCGGGA10500.030.4349925
Produced by FastQC (version 0.11.5)