FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422445
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG6700.042.0847553
CCGAGTA7600.042.070422
CCCGAGT8450.041.199291
GAGTAGC16900.037.8181764
GCTGGGA60300.037.4866039
CCCAAAG43100.037.1121751
AGTAGCT28900.036.424915
TAGCTGG33200.034.821337
AAGTGCT34950.034.2879375
GTAGCTG31200.034.0409936
CCAAAGT39200.033.8255232
TTGGGAG41550.033.2525256
AAAGTGC36400.032.7929654
GTGCTGG38100.032.5632177
CAAAGTG39100.031.850593
CTACTCG5100.031.3465842
AGTGCTG38550.030.4764256
GGAGGCT45450.030.2957789
GCTACTC14500.029.5248811
TACTCGG5150.029.2005563
Produced by FastQC (version 0.11.5)