FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422445
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG7100.048.994523
CTACTCG7750.045.4928052
CCGAGTA6500.042.6699142
GAGTAGC16750.042.376724
GCTACTC18150.041.9621161
CGAGTAG6200.041.7008743
CCCAAAG47050.040.9678541
GCTGGGA67150.040.7386179
AAAGTGC38850.040.5339554
AGTAGCT29000.040.198515
AAGTGCT40450.039.627085
TAGCTGG31600.039.5653427
CCAAAGT43650.039.0935862
GTAGCTG30950.037.3597376
CAAAGTG43900.037.049793
TCGGGAG10300.036.50756
CTACTTG14750.036.3325582
TTGGGAG45400.036.2361076
GGAGGCT53400.036.0007369
AGTGCTG44400.035.99366
Produced by FastQC (version 0.11.5)