FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3610230
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG2107.168358E-571.75856320-329
AGTAGCT68550.057.96315
GTAGCTG71350.056.105276
GAGTAGC45000.055.5161554
CGTTGCG1750.00586291154.808517260-269
CGAGTAG17950.053.3511733
CTACTCG16250.053.1001552
CCGAGTA17950.053.0440442
GCTGGGA151000.052.4698529
TACTCGG17350.052.1106763
TAGCTGG78400.052.0463947
AAGTGCT80500.051.7970165
GCTACTC38050.049.927781
GATCGAT4050.00936451148.680885300-309
AAAGTGC87450.047.952574
CCAAAGT96700.047.139332
AGTGCTG88800.046.620716
TAGGTCG1903.316066E-545.914978340-345
CAAAGTG95050.045.808023
CCCAAAG111350.045.6745031
Produced by FastQC (version 0.11.5)