FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3686453
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGG2857.57737E-467.81843340-345
CTAGTCG1700.003235208863.68793300-309
AGTAGCT64750.056.6645625
GTAGCTG66950.054.448416
CGAGTAG16850.053.117513
GAGTAGC41500.052.989094
GCTGGGA146900.051.8491949
AAGTGCT80250.050.816425
GCCCGTA3851.9658492E-650.20325340-345
TAAATCG3650.00486782849.845646330-339
CCGAGTA18050.049.6083562
TACTCCG3902.2482764E-649.559616340-345
TACCGTG5350.00799168248.737736290-299
TAGCTGG76300.048.1645857
AAAGTGC87050.046.0297474
GTACGTC4207.574118E-546.01964340-345
AGTGCTG89850.045.584866
TACTCGG16600.044.9907383
CTACTCG16150.044.7963262
ATAATCG4106.0436698E-544.473534330-339
Produced by FastQC (version 0.11.5)