FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3428864
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC1100.0030832903191.51562270-279
CTACGAA3459.100765E-5156.29874330-339
ATTTTCG6250.001385258978.83746320-329
GCGAACG2150.001807702673.7529270-279
CGAGTAG14700.057.95513
AGTAGCT59350.057.4180265
TACGAAC4750.0049995357.04773330-339
CCGAGTA15850.056.4319042
TTAACCG3450.00529025656.24396340-345
GAGTAGC41150.052.195594
GTAGCTG64100.052.133616
GCTGGGA128400.051.49179
TAGCTGG66050.050.503637
ATGCCGT12050.00990076647.97726340-345
TTGGGAG86800.047.3468936
AAGTGCT72750.046.9245265
CCCGAGT20900.045.703361
CGTAAAT3950.00792650745.504696330-339
AGTGCTG78800.044.007056
AAAGTGC78300.042.678984
Produced by FastQC (version 0.11.5)