FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3428864
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAA3502.4811015E-9119.58124235-239
ACGCGCG2054.3711232E-4105.423615240-244
TACGCTA1159.463218E-689.50398245
CGAGTAG16050.069.471353
CCGAGTA16750.065.1884162
GAGTAGC39100.062.648774
AAAATCG8109.3477865E-962.25633240-244
AGTAGCT59150.061.923855
GTAGCTG61700.057.8655976
TAGCTGG67100.054.3232777
GCTGGGA136650.053.8573849
TCTACGA3750.006287068653.841698230-234
CGTAAGG3550.006787549252.80997220-224
TACTCGG15900.052.3225363
TTGGGAG95400.052.3217776
CTACTCG15450.051.976822
CCCGAGT21350.051.6843341
AAGTGCT77350.051.4614875
GCTACTC38600.049.9899371
AAATCGT5206.509072E-848.691803240-244
Produced by FastQC (version 0.11.5)