FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638632
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG4850.032.9557232
TACTCGG5350.031.6325473
CTCGGGA6750.027.1605175
GCTGGGA40250.026.8595059
TCGGGAG7600.025.9759676
ACTCGGG7000.025.5194324
GCTACTC12350.025.1239011
GGAGGCT35200.025.104489
CGGAGGT6450.024.7773727
GGGAGGC38500.023.9293778
CCCAAAG38250.023.3524421
AGTAGCT21900.022.7530145
TTGGGAG32550.022.671736
GTCGCCG3350.022.44930894
ACTAGCG1050.002333812422.3850425
TAGCTGG23550.021.7556297
GTAGCTG22400.021.6135086
GTGCTGG27350.020.9671087
TAGGCGG1354.0739562E-420.8927045
CGCGCCC2501.0706572E-820.684562
Produced by FastQC (version 0.11.5)