FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3065760
Sequences flagged as poor quality0
Sequence length35-351
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCG1008.052923E-4299.85062340-345
CGTATCG700.0064382143149.74583290-299
CGCAAGT5008.203238E-489.955185340-345
ATAGCGA3950.001354316579.30159290-299
TACGCAA2454.8371847E-577.32858330-339
AGTAGCT68550.071.7835165
CGAGTAG19800.068.93123
GGACGAT5300.002414559768.54245310-319
GTAGCTG74450.065.930426
AAGTGCT81600.065.778755
CCGAGTA21800.064.605152
GGTCGTA2350.002860129863.798004340-345
GAGTAGC47250.063.5998084
CTACTCG19800.062.4715042
TAGCTGG81650.062.1404657
TACTCGG20650.061.3516733
GCTGGGA166150.060.5220159
AAAGTGC90300.058.08574
CCAAAGT97300.056.954962
AGTGCTG92000.056.7462776
Produced by FastQC (version 0.11.5)