FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3065760
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCG3500.001611189575.90999245
AGTAGCT66700.069.2682655
CTACTCG23150.068.0464862
CGAGTAG18150.067.433243
GAGTAGC42550.067.161064
TACTCGG23400.067.069233
GTAGCTG69900.064.7568056
AAGTGCT82300.064.107385
AGTCGAC1901.4234656E-462.189453235-239
GCTGGGA156900.061.8771869
TTGGGAG107700.061.8742146
CCGAGTA19950.061.6425742
AAAGTGC86950.060.950364
TAGCTGG77600.059.912947
TCGTTAT3100.004600811358.262604240-244
AGTGCTG90350.058.071496
CCAAAGT95750.057.6736262
GGAGGCT132450.057.118869
TCGGGAG29650.057.0763556
CAAAGTG93850.056.9069063
Produced by FastQC (version 0.11.5)