FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7243551
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT164800.069.526195
GTAGCTG166250.066.8708346
CGAGTAG46600.064.958323
CCGAGTA49150.062.3895152
GCTGGGA354050.061.6854069
GAGTAGC118700.060.5258984
TAGCTGG192850.059.132617
AAGTGCT197600.056.770855
TTGGGAG243600.054.34546
AGTGCTG214100.051.9979486
GGAGGCT253750.050.1884549
CCCGAGT65550.048.351341
TCGGGAG51750.047.2738236
GTGCTGG235650.046.7828337
GGGAGGC336150.046.153928
AAAGTGC243600.045.796334
TTTGGGA238950.045.5211525
CTACTCG46850.045.287762
CCAAAGT263450.044.4713862
TACTCGG47950.043.916153
Produced by FastQC (version 0.11.5)