FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796367
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG7850.028.7447433
CTACTCG7950.028.3836842
CCGAGTA9700.023.7475592
CGAGTAG9800.023.5048163
GAGTAGC21000.022.385544
GCTACTC19300.022.1734941
TCGCCGT3051.0913936E-1121.58475794
TCGGGAG11350.021.123656
GCTGGGA63900.021.0395539
AGTAGCT32600.020.4765875
GTAGCTG33850.020.2763046
CCCGAGT12350.020.1817571
CTCGGGA11350.019.4665455
TTGGGAG47100.019.4629346
CCCAAAG43950.019.3673291
GGAGGCT50650.019.2115429
TAGCTGG39550.018.5426887
GGGAGGC59050.018.31068
CTACTTG17200.018.0389042
CCAAAGT42450.017.6081642
Produced by FastQC (version 0.11.5)