FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3297102
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGT1604.473603E-476.11514310-319
GTAACGA2001.2942601E-675.248055340-345
AGTAGCT68550.067.1890265
GTAGCTG73900.062.7437256
AAACGTA7550.00417250459.700737340-345
CGAGTAG18500.058.559753
CCGAGTA19100.057.720532
GCTGGGA160850.057.6917159
AAGTGCT80550.057.3332025
TAGCTGG84100.056.6061027
CGCAGTT7652.1758751E-855.94358310-319
GAGTAGC46950.055.2473344
TTGGGAG101600.054.4724246
AGTGCTG87700.051.882476
GTCGCTA3500.002548651550.49112270-279
TCGAAAG5254.0088175E-449.859776320-329
AAAGTGC91400.049.3758134
TAACGAT3104.5953155E-449.05349340-345
CCAAAGT100400.048.7345662
CAAGTAG34350.048.659763
Produced by FastQC (version 0.11.5)