FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728741
Sequences flagged as poor quality0
Sequence length35-351
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACG800.0065697953162.79825270-279
CGCAATA1606.1955577E-4120.51103330-339
TAAAGCG3006.155617E-496.70823330-339
CGTAAAT3200.002374664960.255516330-339
CGAGTAG13750.056.9341133
CGCCAAA3600.005190026556.511414340-345
CCGAGTA14850.055.045742
AGTAGCT54050.053.4945455
GTAGCTG51350.053.356286
CCCGAGT16650.050.8422471
TAGCTGG56850.050.2852677
GCTGGGA96450.049.9905629
GAGTAGC35500.047.806044
TTGGGAG71000.044.699356
TACTCGG10850.043.1855853
GGAGGCT65650.043.1102459
CTACTCG10850.041.62092
CTCGCGT1200.001630457641.28228160-169
AAGTGCT55450.040.8082855
GGCGTAG3201.4664062E-440.699562270-279
Produced by FastQC (version 0.11.5)