FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728741
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG950.0070031798145.57904215-219
AGTAGCT50650.052.133925
CGAGTAG13650.051.8313523
CCCGACG4400.006907173451.457336245
GTAGCTG50950.051.281196
CTACTCG13450.050.9462552
TACTCGG13350.050.4923443
CCGAGTA13500.050.3449022
GCTGGGA100800.050.071339
TTGGGAG75450.049.7656976
TAGCTGG55750.048.664647
GAGTAGC31950.048.4716074
CCCGAGT15200.047.655041
GGAGGCT78800.047.5784579
GCTACTC32850.046.9843751
CTCGGGA15550.046.573025
CATTACG2450.007045324446.206593245
AAGTGCT53850.044.380595
TCGGGAG16700.043.7002836
GTCTAGT10458.4438734E-443.332497245
Produced by FastQC (version 0.11.5)