FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2465056
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG750.0016194886237.471240-249
GTACCGT2957.9913955E-490.560974240-249
CGAGTAG17850.073.938843
AGTAGCT64800.071.241485
CCGAGTA18250.070.1404342
GTAGCTG68000.068.906776
GAGTAGC44350.066.795144
CAATTCG2350.004266512663.077984330-339
TAGCTGG75900.063.0073287
GCTGGGA134800.062.884669
CCCGAGT20700.061.0428241
CGCGAAC1251.6696446E-460.21749330-339
AAGTGCT63500.059.8118255
TTAGCGG2604.6704322E-457.01279330-339
GCTACTC34250.055.760711
AAAGTGC68150.054.54684
TTGGGAG86700.053.8453456
GGAGGCT88600.053.326289
AGTGCTG71050.052.7428136
CCAAAGT75700.051.9477582
Produced by FastQC (version 0.11.5)