FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2465056
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC650.0032211158188.74307210-214
CGACGTA2700.003604757477.38307245
CGAGTAG17850.076.832343
CCGAGTA18800.074.429552
AGTAGCT63050.073.742755
GAGTAGC41500.071.333514
GTAGCTG65000.070.759996
TACTCGG18100.067.7627943
CTACTCG17900.067.271372
GCTGGGA133900.064.0774469
TAGCTGG72800.064.020947
GCTACTC39600.062.9506951
CCCGAGT22100.061.824691
TTGGGAG98400.060.3394976
AAGTGCT68350.059.8681565
AAAGTGC69600.057.9130174
TCGGGAG22800.057.7064446
AGTGCTG71050.057.1234556
GGAGGCT107450.056.8658189
GCACTTT74400.056.8178061
Produced by FastQC (version 0.11.5)