FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2943528
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7150.033.5870483
GCTGGGA58650.031.7273569
CCGAGTA7600.031.6134052
TACTCGG5550.031.3918153
CGGAGGT8750.030.610727
GAGTAGC19300.030.497234
AGTAGCT32350.030.4214345
CGTCGAG1102.896737E-630.0057751
CTACTCG5500.029.9792022
GTAGCTG30650.029.6017956
TCGCGTT650.00609988528.977065
TAGCTGG34700.028.7086737
GCTACTC15600.028.1098061
CCCAAAG45200.027.957341
GGAGGCT41800.027.4296519
GGCGGAG10800.026.5958425
TTGGGAG44650.026.3214476
AGGCGGA10200.026.3137284
GGGAGGC50450.025.9866188
TCGGGAG7700.025.6418746
Produced by FastQC (version 0.11.5)