FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2943528
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7400.050.1896932
TACTCGG7350.047.9717643
CCGAGTA7050.042.0117342
CTCGGGA9600.040.1543165
CGGAGGT11300.039.5216267
GCTACTC17700.039.3231121
GGCGGAG16150.036.0943155
GCGGAGG13650.035.8170936
CGAGTAG7500.035.729373
TCGGGAG11000.035.4711346
CCCGAGT9300.034.891981
GCTGGGA59700.034.0160839
AGGCGGA17300.032.881464
TTGGGAG48300.031.923936
GGAGGCT47900.031.6005949
GAGTAGC18400.031.1712194
GGAGGTT31300.030.488878
ACTCGGG11900.030.024684
CCCAAAG48900.029.6211051
GGGAGGC61750.029.5382258
Produced by FastQC (version 0.11.5)