FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2684285
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG5500.036.754133
CTACTCG5450.036.2300872
CCGAGTA7450.035.3385162
CGAGTAG6700.033.6795923
CCCGAGT9900.032.7775271
GCTGGGA49550.032.5394639
TTTCGCG600.004157155831.340734
GAGTAGC17400.030.8003754
GGAGGCT39700.030.5484479
CGGAGGT6800.030.4172447
GCTACTC13900.030.1118551
CTCGGGA7600.029.6901135
TTCGCGG804.7770006E-429.3808425
CCGCGTT804.7785643E-429.3791989
TTGGGAG41250.028.9458246
AGTAGCT29250.027.9645355
CTCGCGT856.8090734E-427.6535874
GCGACGT856.812783E-427.651019
GTAGCTG28100.027.6028376
CCCAAAG40250.027.5745471
Produced by FastQC (version 0.11.5)