FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2921287
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG7800.045.2849923
CTACTCG8100.041.3025672
CCGAGTA9600.040.2481882
CGAGTAG9100.038.8157043
CCCGAGT11700.037.8836171
GCTGGGA63750.037.5926369
GTAGCTG34550.035.6481486
GAGTAGC20450.035.4656224
GCTACTC18200.035.2352141
AGTAGCT34800.035.186355
TAGCTGG37200.034.3588567
CGACACG702.1533776E-433.657022
CTCGGGA10200.033.2439425
AAAGTGC38800.031.5588954
CCCAAAG49300.031.3715781
ACTCGGG11500.031.1240394
TTGGGAG51850.031.0977616
CGGAGGT10750.031.0357787
GGAGGCT52150.030.5462679
AGTGCTG41550.029.3030266
Produced by FastQC (version 0.11.5)