FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2921287
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7600.046.3880272
CGAGTAG9250.043.196673
CCGAGTA9550.042.822712
CCCGAGT11550.042.345081
TACTCGG8400.041.9715543
GCTACTC20850.041.7270131
GAGTAGC21500.041.1047944
GCTGGGA70100.039.0268259
AGTAGCT36900.037.7071345
GTAGCTG36500.037.0900846
TAGCTGG40700.036.2654847
GGAGGCT54150.036.1989339
CCCAAAG49350.035.4492761
AAAGTGC41700.033.4806184
TCGGGAG11100.033.45516
CGGAGGT12500.032.7165457
CTCGGGA11800.032.2671975
TTGGGAG55850.032.151296
ACTCGGG11950.031.4698854
GTGCTGG45600.030.9253527
Produced by FastQC (version 0.11.5)