FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2563467
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACAA3950.00197322672.1205245
CGAGTAG16050.071.5055853
CCGAGTA16400.071.010052
AGTAGCT63250.066.8883745
GAGTAGC41150.066.171184
GTAGCTG64500.065.5908056
AATGCGG3801.14269686E-464.99634235-239
CTACTCG18200.063.987082
GCTGGGA129650.063.0031139
TACTCGG18950.062.640923
TAGCTGG70850.062.0943037
GCTACTC39050.061.6798361
CGCAATA1550.003967918560.486652240-244
AAGTGCT68200.060.1362725
CCCGAGT19700.059.1322751
CGTCGTT950.00462591458.64114205-209
TTGGGAG95300.057.5567936
CCCAAAG81800.057.513981
AAAGTGC71700.056.573034
CATCGTT5050.0052232556.411087245
Produced by FastQC (version 0.11.5)