FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458349
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA6550.036.599632
CGAGTAG6500.035.4348373
CGGAGGT8250.033.618047
CTCGCGT702.1842489E-433.5752874
GCTGGGA44600.032.779319
GCTACTC14050.031.798541
TACTCGG5900.031.0713673
TAGGCGG1551.5188562E-930.3260655
CTACTCG5900.030.2746662
GGAGGCT35100.029.999589
CCCGAGT9650.029.7277721
GAGTAGC15100.029.2616424
GGAGGCG11750.029.2033562
GGCGGAG10700.028.9939885
CCCAAAG39950.027.7814641
AGGCGGA12050.027.3060424
GCGGAGG9600.026.931826
TAGCTGG26400.026.353127
GTGCTGG29900.026.2554557
CTCGGGA7350.026.22075
Produced by FastQC (version 0.11.5)