FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2941987
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG906.0560524E-736.661341
CCGAGTA8050.031.5944082
GCTGGGA55100.031.2994449
CGAGTAG7950.030.1985533
CCCGAGT10400.029.4600071
GAGTAGC20150.028.0342714
CTCGCGT856.756032E-427.6907144
GTAGCTG30900.027.3808526
AGTAGCT31200.026.4038075
CTACTCG5600.026.07272
TAGCTGG34650.025.7675137
CCCAAAG44850.025.7487681
TACTCGG5950.025.3172233
GTGCTGG36500.024.3327437
GCTACTC15950.023.9374081
GGAGGCT38600.023.7393869
GGGAGGC44800.023.705698
CCGCTAT1602.0035804E-623.5495362
GGCGGAG9950.022.709175
ACAGGCG12500.022.55645218-19
Produced by FastQC (version 0.11.5)