FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3164612
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG751.4457692E-744.005391
TACTCGG6150.035.224323
CCGAGTA8700.034.6593252
CGAGTAG8300.034.61083
CCCGAGT10550.033.0710751
GAGTAGC20050.031.4738834
CTACTCG6850.030.9514712
CTCGGGA8350.029.891615
GCTGGGA59000.029.6275679
CGTTGCG804.714797E-429.4468882
TCGGGCG650.0060963728.9805455
AGTAGCT34900.028.606875
GTAGCTG34050.028.5778716
CGGAGGT8400.027.9702157
TAGCTGG38950.027.74767
TCGCGTT856.740082E-427.7019925
GGGAGGC51450.026.303428
TTGGGAG46050.026.2349436
CCCAAAG46550.026.1317881
GCTACTC17100.025.9179611
Produced by FastQC (version 0.11.5)