FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3778874
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG857.756171E-944.3516651
CGAGTAG8400.040.909813
TACTCGG7500.040.7977263
CTACTCG7700.036.6968352
CCGAGTA9800.036.522092
GCTGGGA78850.035.6980179
GAGTAGC24000.033.7365844
AGTAGCT43700.032.2087335
CCCGAGT12500.032.044081
GTAGCTG42950.031.8491736
GCTACTC22700.031.1389711
TAGCTGG46950.030.5269327
CCCAAAG65400.029.9746741
CGGAGGT12900.029.870547
TCGCGTT804.7389005E-429.4214345
TTGGGAG61700.028.9512166
GGCGGAG17050.028.4378475
GGAGGCT59300.027.8937479
AAAGTGC51350.027.7770464
GTGCTGG53650.027.5905117
Produced by FastQC (version 0.11.5)