FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3778874
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9750.043.394142
TACTCGG10050.041.162723
CGGAGGT15450.041.0753487
CGAGTAG9500.039.0922743
GCTGGGA82400.037.709459
CCGAGTA10400.037.517852
GCTACTC23400.036.5790751
AGTAGCT42300.036.562615
GGCGGAG21800.036.0115435
TAGCTGG46800.035.155897
GAGTAGC26650.034.926534
GTAGCTG43950.034.7616546
GGAGGCT65450.033.9006739
CCCGAGT14150.033.236921
CCCAAAG63750.032.9027251
GCGGAGG18800.032.5058446
AGGCGGA25050.031.7151224
CTCGGGA13750.031.111465
TTGGGAG71050.031.0302096
GGAGGTT42700.030.4953468
Produced by FastQC (version 0.11.5)