FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2905033
Sequences flagged as poor quality0
Sequence length35-351
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG600.002011161170.6953290-299
TTACGCG600.0073175393143.46954190-199
CGTTAGG1750.0074313083142.69437230-239
ACGCGTG1200.007440318142.65414190-199
TACCGTC3002.2443195E-4124.6116320-329
ACCGACA3603.2761006E-4113.307625340-345
ACGATAC2057.6624093E-491.5399320-329
CCAACGC3109.492187E-680.26808320-329
CCGACAA3500.001445480578.00968340-345
CCGGTTA2251.0963213E-571.13456260-269
TCATCCG6400.003816105761.058014330-339
CCGCAAC3400.003953416460.5329340-345
CTTTCGA4703.6352172E-557.710346340-345
CCTTCGA4900.007656601755.35482340-345
CCGAGTA14000.051.8072622
AGCGGTT4302.0864258E-450.899532300-309
CGAGTAG13400.050.449473
CCCGAGT15200.049.9042931
GAGTAGC30500.049.1555564
AGTAGCT49500.047.028645
Produced by FastQC (version 0.11.5)