FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504041
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG952.0641892E-839.696131
CCGAGTA6200.031.1502022
TACTCGG5000.031.0676563
CGGAGGT6400.029.369617
CTACTCG5400.028.7865052
CGAGTAG6350.028.1691973
GCTGGGA41800.027.5427919
CCCGAGT9000.027.2359541
GAGTAGC15300.026.7660144
CTCGGGA6300.025.4035425
AGTAGCT24900.024.1972795
GGAGGCT32000.023.7893839
GGCGGAG9100.023.2769995
GCGGAGG7650.022.7373896
GGGAGGC34700.022.4800228
GCTACTC12800.022.096481
TAGCTGG27550.022.003227
GTAGCTG26700.021.480726
TCGGGAG6350.021.469636
ACGCGGG1100.00305635521.3686756
Produced by FastQC (version 0.11.5)