FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504041
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA450.001013405641.7843069
CCGAGTA6400.037.4589772
CTACTCG5350.036.9029582
CGAGTAG6550.036.6018683
TACTCGG5550.033.879843
CCCGAGT8450.031.7211321
TCGCGTT953.7495134E-529.6894385
GCTACTC14350.028.8377481
GAGTAGC15250.028.3590934
CGGAGGT6800.027.6524837
TCGGGAG7050.027.3386966
GCTGGGA41450.026.1970949
CTCGGGA7100.025.8214515
ACTCGCG1101.01758684E-425.640883
GGAGGCT32550.025.128359
GTAGCTG25900.024.684386
GGGAGGC37750.024.6564928
TAGCTGG27800.024.5191977
AGTAGCT24350.024.5176665
ACTCGGG8350.021.9559634
Produced by FastQC (version 0.11.5)