FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3014547
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG6650.036.097823
CGGAGGT9200.035.244167
CCCGAGT9900.034.7470741
CCGAGTA7700.033.637282
GAGTAGC18350.032.0631644
CTACTCG5600.031.9554162
GCTGGGA56250.031.745859
CGCGTAA300.004162365631.33334236-37
TAGCTGG33650.030.4436917
TACTCGG5900.030.3154473
CGTTCGG953.7170277E-529.7276134
AGTAGCT31750.029.501345
GGGAGGC54450.029.4294688
GGAGGCT46400.029.0663059
TTGGGAG48850.028.2903526
TCGCGTT1005.2735857E-528.2412325
GGCGGAG11850.028.2015115
GCGGAGG10300.027.8386526
TCGTTCG856.760679E-427.6874853
CCCAAAG45900.027.4112681
Produced by FastQC (version 0.11.5)