FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751046
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG459.98089E-441.9139751
CGTCGAG1750.035.0281071
TCGCGTT753.2409924E-431.3919685
CGAGTAG6650.030.4478473
GCGCGTT404.7848676E-429.37326260-61
CCGAGTA7900.026.2400592
CTCGCGT909.4177277E-426.1599734
GCTGGGA42900.026.0692589
CCCGAGT9800.025.9823841
ACGTCGA1109.965517E-525.7199381
GAGTAGC16350.025.0559754
CCCGAAT3350.023.9284971
CGGAGGT5350.023.7147757
AGTAGCT26700.023.4557955
GGGAGGC38100.023.0635228
GGCGGAG7200.022.8899775
TTGGGAG34750.022.4541446
GGAGGCT35550.022.2064279
ACCGCGC2355.0677045E-922.071721
GTAGCTG26700.022.0060446
Produced by FastQC (version 0.11.5)