FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751046
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG6350.038.49462
TACTCGG6750.035.515733
CCGAGTA6800.031.7994142
GCTACTC15350.030.6356891
GGAGGCT39600.028.725449
TCGGGAG9200.028.612426
CGAGTAG6950.028.4065763
GGGAGGC46100.027.938568
CTCGGGA8900.027.4637495
TTGGGAG39350.027.116626
GCTGGGA46050.026.641429
CCCGAGT9900.025.6504291
CGGGAGG13300.025.0934937
GGCGGAG10700.024.1615855
GTAGCTG26900.024.1146626
CGGAGGT7850.023.9521627
GAGTAGC17800.023.7671384
AGTAGCT28800.023.6658465
TAGCTGG28650.023.6261127
ACTCGGG10600.023.503064
Produced by FastQC (version 0.11.5)