FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3152935
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2550.048.0462761
ACGTCGA1807.2759576E-1231.414871
TACTCGG7350.026.2562663
GTCGAGA4900.024.0263252
CTACTCG7700.023.8515912
CCGAGTA10400.023.0929972
GAGTAGC23350.021.1660044
AGTAGCT36500.020.6330575
CGAGTAG11200.020.5927583
GTAGCTG38550.020.1226796
GCTACTC20250.020.0124361
GCTGGGA72000.019.9717189
TAACGGG1656.786495E-519.9687355
CCCGAGT13750.019.8770431
TAGCTGG41400.019.0693327
CCCAAAG52550.017.9342731
TTGGGAG47850.017.7837316
GGGAGGC57350.017.6989218
CTCGGGA10700.017.5959475
CCGTACA1350.00984618717.4527051
Produced by FastQC (version 0.11.5)