FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3152935
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG10300.027.8386153
CTACTCG10550.027.1785012
CTCGGGA12950.023.593845
CGAGTAG9450.023.3787383
GCTACTC22100.022.7676811
CCTAGCG1252.4160779E-422.5719241
TCGGGAG14300.022.3529476
CCGAGTA9900.022.3157142
GAGTAGC24650.020.2136144
AGTAGCT39050.020.1025185
GTAGCTG40900.019.8831276
GCTGGGA72850.019.808439
CCCAAAG47150.019.4482361
TAGCTGG42450.019.2678597
ACTCGGG15450.018.8633254
GGAGGCT56650.018.5861479
TTGGGAG46950.018.3221916
GGGAGGC64300.017.7646718
CCAAAGT47350.017.1741122
CCCGAGT13850.016.6369481
Produced by FastQC (version 0.11.5)