FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638368
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG5450.037.152263
CTACTCG5400.035.7685552
CCGAGTA6400.035.332352
GAGTAGC17000.033.2388274
CGCGAGT1151.1051816E-732.8056951
GTAGCTG29450.032.4040226
CCCGAAT4400.032.153311
GCTACTC14700.032.08041
CCCGAGT8450.031.8108461
GCTGGGA58100.031.5436539
AGTAGCT30900.031.3922215
TCGCGTA300.004163493431.33153350-51
CGAGTAG6700.030.9236813
TAGCTGG33100.030.6654477
CCCAAAG41650.029.0988081
TCGCGTT650.00609938628.9774365
CCCGGGT5150.028.286427
TTGGGAG42150.027.7708976
GGAGGCT40700.027.4793249
GGGAGGC49600.027.0014258
Produced by FastQC (version 0.11.5)