FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638368
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG6000.050.9227073
GCTACTC17400.046.759361
CTACTCG6900.045.6448252
CGAGTAG7550.042.9587333
TCGCGTT1007.057679E-1042.305025
CCGAGTA7900.040.4620252
CTCGCGT1209.822543E-1139.171314
TCGGGAG9150.038.530626
CCCGAGT10600.038.1561281
GAGTAGC18550.036.9963044
GGAGGCT44950.036.5998659
CTCGGGA9500.036.1200685
AGTAGCT32000.035.5479665
TAGCTGG33300.034.443837
GCTGGGA59400.034.343499
GTAGCTG31950.034.1336676
TCGCGGG702.1842445E-433.575415
TTGGGAG45950.033.350166
ACTCGGG10100.031.1819154
GGGAGGC60950.030.9269078
Produced by FastQC (version 0.11.5)