FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043032
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9600.053.376213
CTACTCG9700.052.340442
GCTACTC21550.045.3928071
CCGAGTA10050.042.565792
GAGTAGC23900.042.2895474
CGAGTAG10400.041.5859833
CCCAAAG57200.039.8764721
TCGGGAG13300.039.586926
GCTGGGA75150.039.282099
AGTAGCT39100.038.8339085
GGAGGCT61750.038.5953459
CTCGGGA12950.038.4787945
ACTCGGG13400.037.1872064
GTAGCTG39250.037.1284946
TTGGGAG59450.036.7693946
TAGCTGG41650.036.456327
AAAGTGC48100.035.477584
CCCGAGT13400.035.096691
CCAAAGT55450.034.5894742
GTGCTGG48400.034.285837
Produced by FastQC (version 0.11.5)