FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2865569
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA2253.232785E-4113.71583320-329
TAGGTCG1756.405404E-493.279465310-319
CGAGTAG16650.066.793923
AACGACG2050.002907666865.42146330-339
CCGAGTA16900.064.480762
GAGTAGC40850.060.5918544
AGTAGCT66400.060.467385
GTAGCTG66700.058.6895456
GCTGGGA129050.057.826839
AAACGAC4252.0647196E-457.671463340-345
TAGCTGG73050.054.8995257
TAGCGGG3408.132829E-753.85473340-345
CGACTAC2803.0422996E-453.32694340-345
CCCGAGT20800.053.241421
AAGTGCT65850.051.7053075
TTGGGAG90850.049.8622636
AGTGCTG70900.049.1048936
CTACTCG13050.048.5283972
GGAGGCT86650.046.217129
CCCAAAG87750.045.406661
Produced by FastQC (version 0.11.5)