FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2865569
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG15100.066.692923
AGTAGCT60350.065.826245
TACTCGG16750.065.105753
CTACTCG17800.062.829232
GTAGCTG63050.062.655466
GAGTAGC38600.061.981094
CCGAGTA16250.061.974192
ACCCGCA5550.003829158661.010605245
TAGCTGG71750.056.376517
GCTGGGA135400.056.0507439
TTGGGAG98000.055.072066
AAGTGCT66900.054.9735035
TCGGGAG21650.052.684356
CTCGGGA20700.052.4134035
AAAGTGC70750.051.824734
GCACTTT79100.051.2217331
CCCGAGT20050.051.074351
AGTGCTG71600.050.899556
TTTGGGA89500.050.6033745
GGAGGCT101200.050.306759
Produced by FastQC (version 0.11.5)