FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3248360
Sequences flagged as poor quality0
Sequence length35-351
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGA3903.9727657E-4107.95533280-289
CGGTACG1008.908211E-488.14966220-229
CAACGAC5550.001126676169.93327340-345
CGAGTAG17900.058.653253
CCGAGTA18450.058.467332
AGTAGCT68150.056.0128335
GTAGCTG68400.055.4035266
AAAGCGC8350.00593813654.60512290-299
GAGTAGC45400.053.540334
GCTGGGA126800.053.2358069
CCCGAGT21850.052.0192871
CTACTCG14150.050.8231962
TACTCGG14350.050.100953
TAGCTGG75200.049.7682387
AGTGCCG6151.9976324E-749.425774290-299
CGGTAGG3500.004787304448.77196310-319
TTGGGAG95100.047.4587826
AACGATA4151.5847664E-446.762615340-345
AAGTGCT73650.044.6248785
GGAGGCT90900.044.24319
Produced by FastQC (version 0.11.5)