FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3248360
Sequences flagged as poor quality0
Sequence length35-251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT950.001380656250.46582245
TCGCGAC1500.007016786109.14927240-244
CGAGTAG15700.061.543953
CCGAGTA16750.059.3445322
TACTCGG18500.059.1330533
CTACTCG19300.057.8337332
AGTAGCT66000.057.8018725
ATCTCGC4250.00475158557.78491240-244
GTAGCTG67700.057.0048566
GAGTAGC41400.056.3349654
GCTACTC42150.056.1299481
GCTGGGA130600.054.378819
TAGCTGG72950.054.3477977
TTGGGAG103550.052.9812976
CCCGAGT19650.050.590981
GGAGGCT106450.050.5452659
CGATCCG1254.071066E-450.277813230-234
GCGTATC1650.001819228449.613308240-244
AAGTGCT70100.048.55925
CTAAGCG2501.025779E-647.588505245
Produced by FastQC (version 0.11.5)