FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017278
Sequences flagged as poor quality0
Sequence length35-351
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAC1150.006394232150.07295330-339
ACGACAA4803.4870746E-4111.529434340-345
AACGAAC3901.5237674E-892.100494340-345
CGAGTAG18000.065.9693453
AGTAGCT68450.064.6367655
CCGAGTA19450.062.5469252
GTAGCTG70500.061.5592276
GAGTAGC46550.060.2161754
TAGCTGG78950.057.1214947
GCTGGGA136250.056.866729
ACGAAAC4700.00574351655.079967330-339
CCTTCGT9750.005806441454.906803340-345
CCCGAGT23900.054.7601741
ACCGCAT3300.00622874654.07488340-345
AAGTGCT69250.048.721325
TTGGGAG91750.048.0475236
AAAGTGC71500.045.750934
AGTGCTG75550.044.913836
GGAGGCT89400.044.3860939
TACTCGG12800.044.1546633
Produced by FastQC (version 0.11.5)