FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017278
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCAA4300.001362538279.17357245
CCGAGTA17950.067.885422
CGAGTAG18700.064.57253
CTACTCG18150.062.2546582
AGTAGCT67450.062.085045
TACTCGG18050.061.067283
GAGTAGC44150.060.9741134
GTAGCTG68700.059.5021066
GCTGGGA133600.058.7030539
CCCGAGT22450.057.9942631
GCTACTC41350.057.4798051
TAGCTGG74450.057.2129487
AAGTGCT70800.055.0790565
TTGGGAG103100.053.2949076
TCGGGAG24550.051.4417886
CTCGGGA22150.051.265745
GGAGGCT106950.051.1140449
AGTGCTG75450.051.0955056
ACGCAAC2350.00845869849.953102240-244
AAAGTGC76550.049.638524
Produced by FastQC (version 0.11.5)