FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3042989
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG3551.0075089E-5106.047066245
GAGCGAT3700.001577649476.310905245
CGATTCG756.759464E-572.29196205-209
AGTAGCT83150.072.076185
CGAGTAG22350.070.956813
CCGAGTA23500.069.927252
GAGTAGC53150.069.731044
TACTCGG23350.069.148373
GTAGCTG84200.068.584146
CTACTCG23550.068.3148962
CCCGAGT25900.064.798811
GCTACTC54400.063.4979171
CGTCGCA1151.3684465E-462.690784205-209
GCTGGGA173750.062.6320049
AAGTGCT84700.061.7344635
TAGCTGG95500.061.3714647
TTGGGAG130450.061.0504466
GGAGGCT136600.060.485239
AAAGTGC90000.058.865134
CGTACAA3250.004441207358.78325240-244
Produced by FastQC (version 0.11.5)