FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3226703
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA700.0022705998212.1444330-339
CGCGTAG1255.2612195E-573.22516250-259
AGTAGCT80900.065.230195
GTAGCTG81050.064.235266
CGAGTAG22050.062.9672623
CCGAGTA22400.061.7466282
GAGTAGC54550.061.473054
ACGCGCA2650.004164007559.75165270-279
GCTGGGA159500.059.1161589
TAGCTGG91550.058.8440867
TTGGGAG110550.054.0042236
CCCGAGT27100.053.897151
AAGTGCT78350.053.612235
CTACTCG16650.051.9632722
TACTCGG16950.051.0221823
AGTGCTG82650.050.194336
GCGCGTA952.8849172E-949.596361
GGAGGCT113250.049.176999
AAAGTGC84550.049.1242334
GCTACTC41550.048.9025461
Produced by FastQC (version 0.11.5)