FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3226703
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG1555.0375797E-4101.733986195-199
CCGTACG1700.005286206577.21223240-244
CGAGTAG20800.070.881793
TACTCGG20300.069.8234943
AGTAGCT76850.069.035165
CTACTCG21600.067.989842
CCGAGTA22400.067.340432
GAGTAGC49250.067.2690054
GTAGCTG81650.065.883076
GCTGGGA161650.062.680999
CCCGAGT25500.062.0760541
TAGCTGG90750.061.5975047
ATACGCG1051.6663957E-460.243965230-234
GCTACTC48700.059.9801941
AAGTGCT81500.058.7403645
ACGCGAG1650.004484599558.647232235-239
TTGGGAG117400.058.427496
ACGACAA5150.004571377858.34809245
CGCCGTA3552.9362677E-456.430546245
GGAGGCT128950.055.3563849
Produced by FastQC (version 0.11.5)