FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3002347
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACC2003.5686814E-4110.933266320-329
CGCGAAC1150.00117294110.177216290-299
TCGAATT4405.278026E-4100.50599320-329
CGAGTAG21700.071.2693943
CCGAGTA23000.066.764172
AGTAGCT81150.066.095825
GTAGCTG81900.065.126186
GAGTAGC54600.062.378684
TAGCTGG89750.060.9636967
CTACTCG17000.060.673142
CCCGAGT26650.059.3873251
TACTCGG17950.059.3751753
GCTGGGA160050.057.8562439
AAGTGCT75450.056.1957325
TTGGGAG104050.054.7649576
GCTACTC40100.054.5036161
GGAGGCT107550.050.927719
AGTGCTG83350.050.87536
AAAGTGC82500.050.129784
CTCGGGA21950.049.8745235
Produced by FastQC (version 0.11.5)