FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260711
Sequences flagged as poor quality0
Sequence length35-251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG500.0053048553159.76707105-109
CGCACGA2050.008228552137.91066245
CGAACAA4104.7092317E-4103.43299245
TATCGGG2250.005063253562.82597245
TACTCGG17550.060.3654173
CGAGTAG15300.059.7525673
CTCGCCG7100.0041638659.72891245
CTACTCG18400.058.744962
CCGAGTA16450.057.8628432
AGTAGCT64550.057.4846535
GTAGCTG65550.056.197496
GAGTAGC40750.056.0867654
GCTACTC40850.054.3855671
GCTGGGA130100.052.948989
TAGCTGG72750.051.0052837
TCGGGAG22700.050.460656
TTGGGAG99550.050.2929956
GGAGGCT106650.050.271299
CCCGAGT19200.050.1507531
CTCGGGA22550.050.080865
Produced by FastQC (version 0.11.5)